HPTLC fingerprint profile and FTIR analysis of hypericin from Hypericum gaitii Haines

Aim: To identify the hypericin content in Hypericum gaitii Haines through HPTLC & FTIR, an endangered medicinal plant and potential for curing many diseases like cancer, AIDS, tumour etc. Methods: Methanolic extract of leaves derived from in vitro raised plants were used in the mobile phase of Toluene: Ethyl acetate: Formic acid (10:8:3) and scanned under UV at 254nm and 366 nm and under visible light. Dried extract powered from leaves were encapsulated in 100 mg of KBr pellet in order to prepare translucent sample discs prepared through applying pressure for FTIR analysis. The data of infrared transmittance was collected over a wave number ranged from 500 to 4000 cm−1. Results: The HPTLC analysis of the methanolic extract has shown several peaks with different Rf values. Five microliter concentrations leaf extract provide 12 spots with hypericin content having Rf value range from 0.65 to 0.68. Conclusion: Both HPTLC and FTIR methods could be used for qualitative and quantitative determination, identification and authentication purposes in order to prevent adulteration in herbal medicines.